| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Sara N (14)
|
MDRAC |
2:35.14 |
2:32.21 |
-2.93 |
| 2 |
Budde, Erin T (14)
|
MDEST |
2:37.69 |
2:35.59 |
-2.10 |
| 3 |
Gude, Lila M (13)
|
MDEST |
2:47.03 |
2:44.01 |
-3.02 |
| 4 |
Friedlander, Ellie S (14)
|
MALAC |
|
2:49.46 |
- |
| 5 |
Hawkins, Jillian E (13)
|
MDEST |
3:02.22 |
2:53.04 |
-9.18 |
| 6 |
Kim, Lindsay S (13)
|
MDUN* |
2:47.98 |
2:53.53 |
5.55 |
| 7 |
Griffin, Lillian E (13)
|
MDEST |
2:51.69 |
2:53.72 |
2.03 |
| 8 |
Cheung, Rachel S (13)
|
MDEST |
3:06.24 |
2:55.84 |
-10.40 |
| 9 |
Yeagle, Carly E (14)
|
MDEST |
3:12.43 |
2:59.57 |
-12.86 |
| 10 |
Carver, Olivia C (13)
|
MDEST |
3:05.06 |
3:00.72 |
-4.34 |
| 11 |
Barnes, Holland A (13)
|
MDEST |
3:05.90 |
3:01.97 |
-3.93 |
| 12 |
Taylor, Emily M (14)
|
MALAC |
3:32.43 |
3:02.37 |
-30.06 |
| 13 |
Hogue, Sofie M (13)
|
MDRAC |
3:02.30 |
3:03.20 |
0.90 |
| 14 |
Rippeon, Genevieve N (13)
|
MDEST |
|
3:05.63 |
- |
| 15 |
Klaus, Bridget E (13)
|
MDEST |
3:18.70 |
3:10.91 |
-7.79 |
| 16 |
Mackey, Andrea (Andi) J (13)
|
MDEST |
3:08.49 |
3:13.88 |
5.39 |
| 17 |
Ekanayake, Anika R (13)
|
MDEST |
|
3:15.01 |
- |
| 18 |
Sane, Shriya A (14)
|
MDEST |
3:28.38 |
3:27.19 |
-1.19 |
| 19 |
Kim, Allison G (14)
|
MDEST |
3:23.58 |
3:32.94 |
9.36 |